Spawn inoculation methods

Ways to start or expand spawn for cultivation.

Spawn inoculation

Adding starter material to a larger batch so it can spread and take over.

1. Prepare: Have clean grain or substrate ready.
2. Add: Mix in a small amount of live culture.
3. Grow: The culture spreads through the new material.

Agar to agar (A2A)

Moving a clean piece from one dish to a fresh dish to keep it growing.

1. Pick: Choose a healthy spot.
2. Transfer: Place it on new agar.
3. Expand: Growth continues in the new dish.

Agar to grain (A2G)

Dropping a clean piece from a dish into sterilized grain so it can spread.

1. Select: Find a clean agar piece.
2. Drop: Place it into grain jar or bag.
3. Colonize: Mycelium grows through the grain.

Grain to grain (G2G)

Mixing colonized grain into fresh grain to multiply the culture.

1. Open: Have both jars ready.
2. Mix: Pour colonized grain into fresh grain.
3. Grow: New grain becomes fully colonized.

Liquid culture to grain (LC2G)

Pouring a small amount of healthy liquid culture into sterilized grain so it can spread quickly.

1. Draw: Take a small syringe of liquid culture.
2. Inject: Add it into the grain jar or bag.
3. Colonize: Mycelium threads spread through the grain.